KMID : 0624620100430050369
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BMB Reports 2010 Volume.43 No. 5 p.369 ~ p.374
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Up-regulation of Idh3¥á causes reduction of neuronal differentiation in PC12 cell
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Cho Sun-A
Seo Min-Ji Ko Je-Yeong Shim Jung-Hee Yoo Jin Kim Jung-Hee Kim Se-Yoon Ryu Na-Kyung Park Eun-Young Lee Han-Woong Lee Yeon-Su Bahk Young-Yil Park Jong-Hoon
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Abstract
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The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to validate Idh3¥á expression by the time course. To identify the function of Idh3¥á in neuronal differentiation stage, the transfection of Idh3¥á to PC12 cells was performed. As a result, we proved that up-regulation of Idh3¥á causes reduction in neural differentiation of PC12 cells. Based on these data, we suggest that Idh3¥á plays a role to the neuronal differentiation.
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KEYWORD
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Idh3¥á, Neuronal differentiation, NGF, PC12 cell, 2-DE
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